Respiratory chain of the alkalophilic bacterium Bacillus firmus RAB and its non-alkalophilic mutant derivative
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چکیده
منابع مشابه
Purification and characterization of the cytochrome oxidase from alkalophilic Bacillus firmus RAB.
A cytochrome oxidase was purified 52-fold from membranes of alkalophilic Bacillus firmus RAB by extraction with Triton X-100, ion-exchange and hydroxyapatite chromatography, and gel filtration. On denaturing gels, the purified enzyme dissociated into two subunits of 56,000 and 40,000 Mr as well as a cytochrome c with an Mr of approximately 14,000. Heme contents calculated for an enzyme with a m...
متن کاملBioenergetic properties and viability of alkalophilic Bacillus firmus RAB as a function of pH and Na+ contents of the incubation medium.
The bioenergetic properties and viability of obligately alkalophilic Bacillus firmus RAB have been examined upon incubation in alkaline and neutral buffers in the presence or absence of added Na+. At pH 10.5, cells incubated in the absence of Na+ exhibited an immediate rise in cytoplasmic pH from less than 9.5 to 10.5, and they lost viability very rapidly. Viability experiments in the presence ...
متن کاملA sodium requirement for growth, solute transport, and pH homeostasis in Bacillus firmus RAB.
Activity of a Na+/H+ antiporter has been suggested to be critically involved in pH homeostasis in obligately alkalophilic bacteria (Krulwich, K. A., Mandel, K. G., Bornstein, R. F., and Guffanti, A. A. (1979) Biochem. Biophys. Res. Commun. 91, 58-62) and in Escherichia coli (Zilberstein, D., Padan, E., and Schuldiner, S. (1980) FEBS Lett. 116, 177-180). A concern with respect to these proposals...
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This study focuses on screening and identification of bacteria, which can produce alkaline xylanase at alkaline pH and high temperature. Bacterial isolates from corncob decaying soil, capable of hydrolyzing xylan were screened. Selected and purified 108 bacterial colonies grown on xylannutrient agar slants were activated and transferred into the fermentation medium. Six highest xylanase produci...
متن کاملIsolation and characterization of mercuric reductase by newly isolated halophilic bacterium, Bacillus firmus MN8
The current study was aimed at isolating and identifying the halophilic and halotolerant bacteria which can produce mercuric reductase in Gavkhuni wetland in Iran. Moreover, tracking and sequencing merA gene and kinetic properties of mercuric reductase in the selected strain were performed in this study. Soil samples were taken from Gavkhuni wetland and cultured in nutrient agar medium...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1983
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.154.1.330-335.1983